A DPC™-Enabled Detection System for Nucleic Acids and Proteins
An important application of Ensemble Discovery’s technology is for in situ generation of chemical signals and development of bioassays that use those signals as research tools, diagnostic products, and molecular imaging agents. Ensemble is developing methods for the sensitive detection of both nucleic acids and proteins. Analyte-recognition elements can be DNA-based (for nucleic acid detection) or antibodies, aptamers or small molecules for protein detection.
DPC™ probes are unique in that they do not generate a signal until two complementary probes, each recognizing a different target element, bind to their targets. This is based on a single DPC reaction as shown below.
For recognition of a biological analyte, the DPC signal generation strands are linked to elements that recognize that analyte specifically. These target elements can be, for example, adjacent regions on a DNA target (nucleic acid probe), or different proteins in a protein complex (protein probe). Nucleic acid DPC probes can be used for detection of specific genomes or genomic variations and are useful in homogeneous detection assays. Protein DPC probes are useful for detection of “functional” information about cell signaling events by detecting key changes in individual proteins, such as how they are modified (post-translational modification) or how they interact with other proteins in complexes. Detecting such cell signaling changes in diseases such as cancer, Alzheimer’s Disease, and inflammatory diseases will provide critical information of value to researchers and physicians.
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