AURKB Antibody FITC conjugated

Volume : 50 µg
Clone Number :
Aliases : AIK2 antibody; AIM-1 antibody; AIM1 antibody; ARK-2 antibody; ARK2 antibody; AurB antibody; AURKB antibody; AURKB_HUMAN antibody; Aurora 1 antibody; Aurora and Ipl1 like midbody associated protein 1 antibody; Aurora kinase B antibody; Aurora related kinase 2 antibody; Aurora- and Ipl1-like midbody-associated protein 1 antibody; Aurora-B antibody; Aurora-related kinase 2 antibody; Aurora/IPL1 related kinase 2 antibody; Aurora/IPL1-related kinase 2 antibody; IPL1 antibody; PPP1R48 antibody; Protein phosphatase 1 reg µLatory subunit 48 antibody; Serine/theronine kinase 12 antibody; Serine/threonine protein kinase 12 antibody; Serine/threonine-protein kinase 12 antibody; Serine/threonine-protein kinase aurora-B antibody; STK-1 antibody; STK1 antibody; STK12 antibody; STK5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q96GD4
Immunogen : Recombinant Human Aurora kinase B protein (1-255AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key reg µLator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtub µLe stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtub µLes to kinetochores and is a key reg µLator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercell µLar chromosome bridges and accum µLation of DNA damage : phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed : 22422861 PubMed : 24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1 SEPT1 VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at \'Ser-10\' and \'Ser-28\' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively reg µLates its transcriptional activity. Key reg µLator of active promoters in resting B- and T-lymphocytes : acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : AURKB
Research Areas : Epigenetics and Nuclear Signaling; Cancer; Cell biology; Signal transduction